Journal: Cancer Medicine

Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

doi: 10.1002/cam4.72232

Figure Lengend Snippet: In vivo and ex vivo validation of TNBC targeting by Dextran‐AF680. (A) The average radiant efficiency of Dextran‐AF680 peaked at 3 h after intravenous injection, with significant differences between 1 h and 3 h and between 3 h and 6 h ( p = 0.0313, n = 6) by in vivo fluorescence imaging. (B–D) The biodistribution of Dextran‐AF680 in orthotopic models was monitored using conventional fluorescence imaging at 3, 8, and 24 h. At each time point, mice were euthanized, and tumors and organs were harvested for ex vivo fluorescence imaging. Ex vivo organ imaging revealed an increasing trend in Dextran‐AF680 uptake by TNBC tumors, the liver, and the kidneys ( p = 0.0022, p = 0.0043, n = 6). (E, F) Tumors were cryo‐sectioned, stained, and imaged on a confocal microscope (blue, Hoechst; red, Dextran‐AF680; green, F4/80‐AF488; scale bars, 25 μm) Strong, well‐demarcated fluorescent signal was visible ex vivo, showing a high degree of overlap between the F4/80 targeted pan macrophages and Dextran‐targeted cells. Furthermore, quantification of fluorescent signals confirmed a clear signal from intravenously injected Dextran, further supporting its utility as an imaging probe for in vivo targeting of M2 macrophages. (G, H) Dextran‐targeted cells (red) did not overlap with CD86‐positive M1‐like macrophages (green). Data are presented as the mean ± standard deviation (SD).

Article Snippet: Bioluminescence signals were acquired 10 min post‐injection using an IVIS Spectrum CT system (PerkinElmer, USA).

Techniques: In Vivo, Ex Vivo, Biomarker Discovery, Injection, Fluorescence, Imaging, Staining, Confocal, Microscopy, Standard Deviation

Journal: Advanced Science

Article Title: Spatially Confined/Ligand‐Directed Ultrabright NIR‐AIE Nanoprobe for In Vitro Rapid Detection and In Situ Imaging of Bacterial Septic Arthritis

doi: 10.1002/advs.78156

Figure Lengend Snippet: Schematic design and functional applications of PPANPs in bacterial diagnosis and therapy. (a) Schematic illustration of the confined co‐assembly strategy for constructing ultrabright NIR fluorescent nanoparticles (PPANPs) via co‐embedding PbS QDs and AIEgens in PMAO. (b) In vitro bacterial detection using PPANPs‐based NIR‐LFA in synovial fluid. (c) In situ NIR imaging diagnosis of septic arthritis; inset shows photothermal bacterial eradication in vitro.

Article Snippet: In vivo fluorescence imaging was performed using an IVIS Lumina Series III small animal imaging system (PerkinElmer, USA).

Techniques: Functional Assay, Biomarker Discovery, In Vitro, In Situ, Imaging